A decade ago, biological
indicator (BIs) used a visual readout method to determine if a
sterilization cycle successfully inactivated microorganisms. Because these antiquated BIs took one to seven days for growth readout results, they were an untimely indicator of potential catastrophic sterilization failures.
In recent years, the time to read for BIs drastically improved with the introduction of
fluorescence readout. Today, the fastest time to results in the market is the STERRAD VELOCITY®
System with a 15-minute* readout.
For information on biological indicator systems and process challenge devices, read our
Process Monitoring – Biological Indicators and Process Challenge Devices (PCD) for
- α-MUG: a fluorogenic dye that becomes fluorescent upon
with α-glucosidase (AG)
- α-glucosidase (AG): an enzyme naturally produced by spores
When the STERRAD VELOCITY® BI/PCD ampule is crushed, the surviving microorganisms containing
α-glucosidase (AG) are mixed with media that contains α-MUG. The enzymatic reaction splits α-MUG
to 4-MU, a fluorescent compound, and Glucose.
If organisms survive after the sterilization cycle, the media will fluoresce, indicating a positive result. Conversely,
if there are no surviving microorganisms after the sterilization cycle, the media will not
fluoresce, and the BI is considered negative.
This advancement in technology enables sterile processing leaders to establish better balance between keeping up with clinical demand and confidence of BI/PCD results before releasing instruments. Fast time-to-read increases throughput for the busiest sterile processing departments and operating room.
In addition to sophisticated rapid-read technology, STERRAD
VELOCITY® Biological Indicator System also has a unique connection to STERRAD®
Systems via ASP ACCESS® Technology to
enhance compliance through BI reminders per hospital policy, greater adherence to device IFUs, and
improved record keeping. Via ASP ACCESS® Technology, the STERRAD VELOCITY®
Reader integrates with STERRAD® Sterilizers to provide a guided, error-resistant workflow with
automatically correlated sterilizer and BI record to ensure consistent and complete BI records
that are AAMI compliant. This provides the confidence in audit-ready BI records so that the focus is on
Learn more about ANSI/AAMI standards for low-temperature sterilization: Importance of Following
Industry Standards and Guidelines
For more information on STERRAD VELOCITY® BI/PCD rapid-read technology or automated
recording-keeping ability, get a consultation from a sterilization expert.
*15 or 30 minutes dependent on software version. Refer to the IFU for actual time to results.